Isfahan dental student's knowledge and attituale about research

Scientific development is essential for each type of development. The first step for planning is to know present situation. For planning to improve student role in research, we must know how much do they know about research methodology and what kind of attitude they have about it. The aim of this study was to determine Isfahan dental students' knowledge and attitude about research. All Isfahan dental school students [calss of 1999-2003] participated in this cross-sectional descriptive-qualitative study [n=300]. Data was collected by self-administered questionnaire consisting of three parts: knowledge, attitude and demographic questions. The content validity of question-naire was confirmed by experts and its reliability by split half method. The face validity was also checked. The data were analyzed by SPSS-11 soft ware using frequency distribution and mean and correlation coefficient list. The mean mark of students' knowledge was 2.09 +/- 1.92 out of 10. Which would be increased with inereasing level of training and education. Ninty four persent of students would absulotely agree with necessity of participation in research activity during heir study period, 23.5 percent by increasing quality of services to the patient, 25.4 persent by increasing quality of study skills, 46.3 percent were in agreement with university support for research activity and only 15.1 percent of students believed that participating in research activity is wast of time. According to the results of this study, student's research knowledge is very low in spite of their attendance in research methodology workshops [40.5 persent]. Because of their desire and positive attitude toward research activity, faculty officials should plan in more diciplined way, to improve the students' skills and knowledge

Effect of human follicular fluid on implantation of mouse embryo

Prevention of implantation is considered as one of the suitable methods for contraception or interception. Therefore during this study the effect of human follicular fluid [FF] on mouse implantation was evaluated. The results obtained during this study show that FF contains enzymes and upon incubation with mouse uterine tissue, results in delimitation of epithelium. This effect was inhibited by heat inactivation or addition of EDTA to FF. In vivo uterine wash with follicular fluid on days 3,4,5 and 6-post mating prevents implantation and therefore significantly reduces implantation and pregnancy rate. However uterine wash on day 7 post mating with FF had no effect on implantation and pregnancy rate. On day 3,4,5,6 and 7 with Ham's 10 as control had no effect on implantation or pregnancy rate. Addition of EDTA also prevented the in vivo effect of FF, suggesting that the active agent present in FF is likely to be a metalloprotoinase which inactivates with heat and addition of EDTA. Taking into consideration the fact that FF does not affect the pregnancy of the next cycles, thus the FF or its active agent can be considered as good interceptive agent for prevention of pregnancy rate